The draconian measure comes after French officials revealed those looking to get across the Channel were arriving in the port town at a rate of 100-a-week.
Three rest areas on the A26 autoroute and one on the A16 have been sealed off to halt the increasing flow in northern France.
A regional official said: “The reason for the closures is to prevent intrusions into lorries heading to the coast.
“People smuggling gangs are known to use these service stations to help migrants get aboard vehicles that are mostly destined for the UK.”
The closures come a month after it emerged hundreds of migrants had returned to secret camps after the notorious “Jungle” was flattened.
The emergence of at least six rural settlements in the Nord-Pas-de-Calais region has raised fears of a new border crisis.
Officials thought the mayhem of the past two years had ended in October when the Jungle was bulldozed and 10,000 migrants dispersed.
Yet dozens of service stations on motorways leading to the port and Eurotunnel terminals have become the new frontline in the fight against people smuggling with lorry drivers describing them as “no-go areas” after dark.
British trucker Graham Gammond, 65, from Hereford, said: “The problem is now moving further away from Calais. It’s a daily risk and I dare not stop near the port because it’s now so dangerous. The situation has become unreal.”
Around 150 refugees are huddled in a shantytown in woods close to the village of Norrent-Fontes, around 30 miles south east of Calais.
Their squalid settlement is a short walk to the Saint Hilaire Cottes services on the A26 to Calais which has become a magnet for trafficking gangs.
Earlier this month Calais police arrested five hotel owners in the town for harbouring illegal immigrants and offering them “guaranteed passage” to the UK for up to £9,000 each.